Inverted Microscope –
for all Routine and Laboratory Applications
Leica DMIL – compact invertedmicroscope for laboratory routine
The new inverted Leica microscope blends ergonomy, a compactdesign and effective contrasting methods into a system for virtuallyunlimited life science applications.
The integration of Leica HCS* optics extends the range of objectivesfor inverted microscopy.
For the first time, high-quality relief contrast can be producedwithout special objectives with our new Integrated ModulationContrast (IMC) technique.
Optimised phase contrast and brilliant incident light fluorescencemake the Leica DMIL the number one choice in contrastingmicroscopes.
Leica Design by Ernest Igl/Christophe Apothéloz
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With its unbeatable modularity, ergonomy and free view of thespecimen, teamed with newly developed and optimised contrastingtechniques, the Leica DMIL offers you a top level introduction toinverted microscopy.
The adaption of the Leica DMIL to infinity optics allows theintegration of Leica HCS* components for superb image resolution,brilliant contrast and precise colour rendering. The DMIL is theinverted equivalent of our successful upright DM microscopes ofthe L and R class.
The Leica DMIL is a microscope for all applications in microbiologyor the cell culture laboratory. A universal inverted microscope forroutine use: stable and space-saving, flexible and upgradablewith optics from Leica’s research microscopes.
* Harmonic Component System
Microinjection of oocytes in mouse
RNA microinjection of frog oocytes(Xenopus)
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The Leica DMIL contrasting microscope
Discussion unit4
The System
The stand
The stand of the Leica DMIL microscope is stable, aluminium-cast and excellently designed. There are two versions for biologicalapplications:
The DMIL for brightfield, phase and modulation contrastand
the DMIL with additional incident light fluorescence unit.Appreciated by users for many years, the stable T-shaped micro-scope base offers plenty of valuable space round the microscopeand ensures comfortable, fatigue-free microscopy. The micro-scope’s footprint is optimised to provide the necessary room forexperiments, and all controls are ergonomically located. Manydifferent components can be adapted to suit individual require-ments. The high stability, low centre of gravity and four vibrationdamping feet eliminate vibrations even in extreme conditions. Theexcellent stability of the DMIL also makes it the ideal solution forphotography with long exposure times. In addition, finite elementcalculations and thorough practical testing in a wide variety ofapplications guarantee focusing which is not only ultra precisebut also stable over long periods of time.
Cardiac muscle cells
Due to its modularity, the Leica DMIL is particularly suitable forliving cell microscopy.
Its modern, practical design, the integration of state-of-the-art,top quality optics and the excellent standard of the adaptedcontrasting techniques prove useful in research tasks as well asroutine applications.
You will be convinced by the DMIL’s first-class technology andmany innovative and practical ideas.
We at Leica believe microscopy should be a pleasurable experienceand designed the DMIL to be associated with enjoyment at thelaboratory workplace.
DMIL with illumination column the other way roundTrinocular tube with MPS
Trinocular tube with DC100
Ergonomic phototube
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The Technology
Nosepiece focusing
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Samples are focused with the quadruple objective nosepiece.The reliability, stability and precision of the focusing is not influencedby the microscope stage and the samples on it or by accessorycomponents such as the object guide and manipulators.Illumination system
The compact brightfield illumination unit is attached to a columnand can be comfortably adjusted. Setting the correct height forthe condenser used or the specimen on the stage is facilitated bymarkings along the column. The pre-centred, extremely powerful6V 35W halogen lamp provides optimal illumination even of criticalspecimens. The transmitted light illumination concept is roundedoff by the integration of the contrast slide (for phase and modulationcontrast), the module for light filters with 32 mm diameter and theaperture diaphragm. The microscope stage with illumination armcan be turned round by 180°and is freely accessable for samplepositioning from three sides.
Light filters
The filter module on the illumination column accommodates 32 mmdiameter filters in a spoon-shaped holder. We are constantlyadding to our wide range of filters, enabling you to selectivelyoptimise the illumination for observation and image documentation.Built-in 6V 35W power supply
The 6V 35W power supply, which provides the full lamp powerincluding on/off switch, status indicator and brightness adjustment,is fully integrated in the microscope stand. Apart from theergonomic advantages, this saves space on the microscope deskand allows the microscope to be easily picked up as a unit andmoved elsewhere.
Condensers
The Leica DMIL offers you a choice of two condensers.
The S 90/0.23 condenser with a free working distance of 90 mmand a numerical aperture of 0.23 is designed for brightfield andphase contrast and is particularly suitable for specimens in bulkylaboratory vessels.
The S 55/0.35 condenser with a free working distance of 55 mmand a numerical aperture of 0.35 for bulky containers is designedfor brightfield, phase and integrated modulation contrast (IMC)and is particularly suitable for higher magnifications or thickspecimens.
Without a condenser, the maximum free working distance is 200 mm.Stage and accessories
The DMIL offers a wide variety of stages with a whole array ofaccessories and different inserts for your specimen vessels:The standard stage is a fixed stage plate of 252 x 212 mm. The stagecan be widened by 70 mm on both sides by adapter plates. Theinterchangeable stage inserts (20-50 mm) allow smaller petridishes to be used as well without losing the focus when theobjective nosepiece is rotated.
Object guides can be attached to both the left and right of thestage and have a minimum adjustment range of 83 x 127 mm. Thecontrol of the coaxial drive is in an ergonomically low position sothat you can rest your hands on the desk while scanning specimens.The object guides accommodate special and multi-purpose framesfor all types of culture vessels.
A heating stage up to max. 45°C, a 3-plate mechanical stage andscanning stages round off the range of stages for the DMIL.
Micromanipulation ...
... and scanning stage7
Eyepieces and objectives
DMLB tube
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The Optics
The optics are the heart of every microscope and decisive for thequality of information. We have set new standards here by intro-ducing our HC optics.
The Leica DMIL is designed for brightfield, phase contrast, Inte-grated Modulation Contrast (IMC) and incident light fluorescence.All infinity-corrected high performance objectives in the Leicarange with 25 mm screw thread are compatible with the DMILmicroscope.
Even earlier-type Leica objectives can be adapted for use on theDMIL. We offer a wide range of special objectives for invertedmicroscopy applications with long free working distances(L objectives) and/or with correction mounts (Corr objectives) tocompensate for different vessel thicknesses. The latest Leicaoptics brochure features our whole range of objectives.Depending on the tube configuration, there is a wide choice ofeyepieces with magnifications 10x, 12.5x, 15x, 16x or 25x, suitablefor fields of view up to 20 mm. Besides special high-point eyepiecesfor eyeglass wearers, we also supply eyepieces with adjustableeyelens (M eyepieces), into which different types of graticule canbe inserted.
The DMIL range also comprises many different observation andphoto tubes. The tubes are interchangeable and can be individuallyrotated by 360°in the tube mount and then fixed in position. Alltubes are fitted with an infinity tube lens 1x.
The following tubes are used on the DMIL:
•Binocular tube ILB, with 45°viewing angle, for eyepieces with23.2 mm outer diameter
•Trinocular (photo)tube ILT, with 45°viewing angle, for eyepieceswith 23.2 mm outer diameter, with vertical photo/TV exit withswitchable light path for either 100% visual or 100% photo/TV.The position of the photo/TV exit 88 mm to the side of the tubehas the special advantage that it allows an unobstructed viewof the specimen.
Other tubes from the Leica DML rangecan be used via an IL/L adapter:•Binocular tube HC LB45°viewing angle•Binocular tube HC LVB0-35°ergotube•Trinocular (photo)tube HC L1T45°viewing angle•Trinocular (photo)tube HC L3T45°viewing angle•Trinocular (photo)tube HC LV1T0-35°ergotube
Developments in diagnostics and med./biol. research (e.g. fluores-cence applications) and the increasing use of video technology andelectronic image processing have to be paralleled by intelligenttechnical adaptations of the microscope system.
The new HCS optics concept introduced with the Leica DMRmicroscope meets this requirement. It is the result of an integralsystem consideration, harnessing all technological potential.The abbreviation HCS stands for Harmonic Component System.Its special features are:
•well-balanced optical and mechanical fitting dimensions
•harmonious balance of all optical system components, i.e. theparameters contributing to the microscope’s performance (objectives, tube lenses, tubes, eyepieces, TV cameras/adapters,etc.) have been harmonised throughout the entire optic system.This has created scope for even greater optical opportunities.The HCS system is the answer to your application requirementsnot only today, but in future, too.
Rat testicles, IMC
ApplicationFITC351+364 mn UVAr488mn568mn647mn Ar/Kr490520TRITC557576CY53504004505005506006486706507009
Spinal cord, cat
Lymphocyte toxicity test
double staining, strongly positive
Lymphocyte toxicity test
double staining, weakly positive
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Contrasting
The Leica DMIL is the microscope for all requirements in the cellculture lab. A universal inverted microscope for routine application:stable and space-saving, flexible and upgradable with optics fromLeica research microscopes.
Brightfield
The whole range of objectives from 4x-100x magnification can beused for brightfield applications. Samples in almost any kind ofvessel can be examined with or without a condenser, while a 6V35W halogen lamp ensures optimal illumination.
Phase contrast
In vivo/ in vitro microscopy specimens are mostly living culturesor microorganisms and are examined under sterile conditions.Contrast of the transparent tissue can only be enhanced by opticalmethods.
Phase contrast is a useful technique for high-contrast imaging ofunstained specimens. The phase contrast technique used byLeica on the DMIL has been optimised for inverted microscopyapplications and produces equally excellent contrast in waterysolutions and of dry preparations in petri dishes.
IMC (Integrated Modulation Contrast)
The innovative technique of Integrated Modulation Contrast (IMC)now introduced by Leica in the DMIL is based on Hoffman’sprinciple and produces this contrast without the need for specialobjectives–ordinary brightfield or phase contrast objectives canbe used. The Leica IMC provides a high-contrast, 3D image oftransparent objects similar to that of interference contrast. Plasticculture vessels do not impair the quality of the image as thetechnique is polarisation-neutral.
The diaphragm slide on the side of the illumination and theswitchable modulator in the intermediate image of the pupilproduce the type of contrast named after Hoffman without modi-fying the objectives. High contrast, high resolution, a halo-freerelief image of either stained or unstained specimens make Leica’sIMC a new standard in the class of inverted routine microscopy.Fluorescence
The fluorescence model of the DMIL reflects the growing signifi-cance of fluorescence for in vivo/in vitro microscopy. The maincomponents of this configuration are an incident light axis integrat-edin the microscope stand, incorporating a fluorescence slide forthree filter cubes. A wide range of light sources with multi-lens,chromatically corrected collectors brighten up even the weakestfluorescence. The fluorescence filter cubes comprise an optimallymatched combination of excitation, reflection, band-pass andbarrier filters. We are constantly updating our range of filtercubes to keep pace with the latest challenges in biology andmedicine.
Transmitted light techniques can be used simultaneously or inalternation in order to clearly allocate fluorescent and non-fluorescent structures.
The Leica DMIL offers you a powerful system for immunology,cytopathology, virology – in fact wherever fluorescence techniquesare used in combination with inverted microscopy.
Human brain
Femtotip microinjection needle(Photo: Eppendorf)
Fibroblasts
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Leica Microsystems – the brandfor outstanding products
Leica Microsystems’ Mission is to be the world’s first-choice provider of innovativesolutions to our customers’ needs for vision, measurement, lithography and analysisof microstructures.
Leica, the leading brand for microscopes and scientific instruments, has developedfrom five brand names, all with along tradition: Wild, Leitz, Reichert, Jung andCambridgeInstruments. Leica symbolizes not only tradition, but also innovation.
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