FLUORESCENCE OBSERVATION DEVICE, FLUORESCENCE OBSE

专利名称：FLUORESCENCE OBSERVATION DEVICE,

FLUORESCENCE OBSERVATION SYSTEMAND FLUORESCENT LIGHT IMAGEPROCESSING METHOD

发明人：ISHIHARA, YASUSHIGE申请号：EP11868025申请日：20110621公开号：EP2724657A4公开日：20140430

摘要：To sufficiently remove dependency on distance and the like that remain in animage subjected to dividing operation and to perform observation with fluorescenceimages that are high in quantitativity. There is provided a fluorescence observationapparatus (1), including: a lighting section (4) including a light source (3) that emitsillumination light and excitation light; a fluorescence imaging section (18) adapted to pickup fluorescence generated on a subject (A) and to acquire a fluorescence image (G2); areturned light imaging section (17) adapted to pick up returned light returned from thesubject A and to acquire a returned light image (G1); a preprocessing section (23) adaptedto multiply at least either the fluorescence image (G2) or the returned light image (G1)by a coefficient in which a distance property of fluorescence intensity and a distanceproperty of returned light intensity, which are acquired in advance with respect to astandard sample, come to be in a relation directly proportional to each other and togenerate a fluorescence image for correction (G2'') and a returned light image forcorrection (G1''); and a fluorescence image correction section (24) adapted to divide the

fluorescence image for correction (G2''), which was generated by the preprocessingsection (23), by the returned light image for correction (G1'').

申请人：OLYMPUS CORPORATION

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